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| A Homolog of the Cell Apoptosis Susceptibility Gene Involved in Ovary Development of Chinese Shrimp Fenneropenaeus chinensis | |
Wen, Rong1,2; Li, Fuhua1; Xie, Yusu1; Li, Shihao1,2; Xiang, Jianhai1 ; Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China.
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| 2012 | |
| 发表期刊 | BIOLOGY OF REPRODUCTION
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| ISSN | 0006-3363 |
| 卷号 | 86期号:1 |
| 文章类型 | Article |
| 摘要 | The cell apoptosis susceptibility (CAS) gene is a homolog of the yeast chromosome segregation (CSE1) gene, which functions in cell proliferation and apoptosis. In the present study, a homolog of CAS was cloned from Chinese shrimp Fenneropenaeus chinensis (FcCAS). The full-length FcCAS cDNA is 3534 bp and contains an open reading frame encoding 968 amino acids. The predicted tertiary FcCAS structure is highly similar to that of CSE1 from the yeast Saccharomyces cerevisiae. RT-PCR analysis showed that the FcCAS gene is expressed mainly in testis, ovary, stomach, lymphoid organs, gills, and hemocytes. RNA in situ hybridization showed that FcCAS transcripts were distributed mainly in the cytoplasm of oocytes. Western blot analysis showed that FcCAS could be detected only in testis and ovary, and its expression levels differed at different developmental stages of ovaries. Immunohistochemical analysis showed that FcCAS existed in both the cytoplasm and the nucleus, which suggested that FcCAS might function as a nuclear protein. No transcript was detected in the abnormally developed ovaries of triploid shrimp. Therefore, we inferred that the FcCAS gene might be one of the key genes that is closely related to ovary development in shrimp. |
| 关键词 | Cas Gene Expression Invertebrates Oocyte Ovary Ovary Development Shrimp |
| 学科领域 | Reproductive Biology |
| DOI | 10.1095/biolreprod.111.092635 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000300548400006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12258 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Li, FH (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wen, Rong,Li, Fuhua,Xie, Yusu,et al. A Homolog of the Cell Apoptosis Susceptibility Gene Involved in Ovary Development of Chinese Shrimp Fenneropenaeus chinensis[J]. BIOLOGY OF REPRODUCTION,2012,86(1). |
| APA | Wen, Rong,Li, Fuhua,Xie, Yusu,Li, Shihao,Xiang, Jianhai,&Li, FH .(2012).A Homolog of the Cell Apoptosis Susceptibility Gene Involved in Ovary Development of Chinese Shrimp Fenneropenaeus chinensis.BIOLOGY OF REPRODUCTION,86(1). |
| MLA | Wen, Rong,et al."A Homolog of the Cell Apoptosis Susceptibility Gene Involved in Ovary Development of Chinese Shrimp Fenneropenaeus chinensis".BIOLOGY OF REPRODUCTION 86.1(2012). |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A Homolog of the Cel（469KB） | 限制开放 | 使用许可 | 浏览 | |||
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