| Molecular cloning and characterization of the myostatin gene in a cultivated variety of bay scallop, Argopecten irradians | |
Guo, Lin1,2 ; Li, Li1; Zhang, Shoudu1,2; Zhang, Guofan1; Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
| |
| 2012-06-20 | |
| 发表期刊 | AQUACULTURE
 |
| ISSN | 0044-8486 |
| 卷号 | 350页码:192-199 |
| 文章类型 | Article |
| 摘要 | In this study, we cloned and characterized the myostatin (MSTN) gene from a cultivated strain of bay scallop, Argopecten irradians, "Zhongkehong", and named it as the Ai-MSTN gene. The Ai-MSTN cDNA sequence was 3553 bp, contained three exons with 1380 bp in the open reading frame encoding 459 amino acids, and was 2014 bp longer than one previously reported. Analysis of its genomic structure and expression profiles indicated that the sequence of MSTN was 12473 bp (Genbank: JN684902) containing 3 exons and 2 introns, which was conserved with its vertebrate and invertebrate homologs. In silico analysis revealed that the promoter region of Ai-MSTN contained several muscle specific regulatory elements. The 3D structure of Ai-MSTN was similar to that of the known mouse MSTN. Ai-MSTN was expressed not only in the digestive gland, pericardium, gill, adductor muscle, gonad and mantle of the bay scallop, but also in its hemolymph, which was firstly reported in mollusks. These findings provide genomic and protein information and expression profiles of the Ai-MSTN gene and lay the groundwork for further investigations of its functional significance in this economically important sea food, bay scallops. (C) 2012 Elsevier B.V. All rights reserved. |
| 关键词 | Myostatin Bay Scallop Argopecten Irradians "zhongkehong" |
| 学科领域 | Fisheries ; Marine & Freshwater Biology |
| DOI | 10.1016/j.aquaculture.2012.03.040 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000305068800027 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12235 |
| 专题 | 海洋生物技术研发中心 文献信息中心 |
| 通讯作者 | Zhang, GF (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Guo, Lin,Li, Li,Zhang, Shoudu,et al. Molecular cloning and characterization of the myostatin gene in a cultivated variety of bay scallop, Argopecten irradians[J]. AQUACULTURE,2012,350:192-199. |
| APA | Guo, Lin,Li, Li,Zhang, Shoudu,Zhang, Guofan,&Zhang, GF .(2012).Molecular cloning and characterization of the myostatin gene in a cultivated variety of bay scallop, Argopecten irradians.AQUACULTURE,350,192-199. |
| MLA | Guo, Lin,et al."Molecular cloning and characterization of the myostatin gene in a cultivated variety of bay scallop, Argopecten irradians".AQUACULTURE 350(2012):192-199. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Molecular cloning an（1789KB） | 限制开放 | 使用许可 | 浏览 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论