| Isolation, identification and characterization of a novel antioxidant protein from the nematocyst of the jellyfish Stomolophus meleagris | |
Li, Rongfeng1,2; Yu, Huahua1 ; Xing, Ronge1; Liu, Song1; Qing, Yukun1,2; Li, Kecheng1,2; Li, Bing1,2; Meng, Xiangtao1,2; Cui, Jinhui1,2; Li, Pengcheng1; Yu, HH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
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| 2012-10-01 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| ISSN | 0141-8130 |
| 卷号 | 51期号:3页码:274-278 |
| 文章类型 | Article |
| 摘要 | Reactive oxygen species (ROS) can damage the lipids, proteins and DNA when produced excessively in cells. Here, we describe the isolation and identification of a novel antioxidant protein named SmP90 from the nematocyst of jellyfish Stomolophus meleagris by 50% ammonium sulfate precipitation and gel filtration chromatography, superdex75. HPLC and SDS-PAGE analysis revealed >95% purity of SmP90 with apparent molecular weight of 90 kDa, approximately. The identification of SmP90 was confirmed by both N-terminal amino acids sequencing, with the sequences of NLDTPYCFYSGDYGG, and peptide mass fingerprint (PMF) analysis by MALDI-TOF-MS. However, no known protein had been completely matched in the database, which indicated that SmP90 might be a novel protein. The antioxidant assay result showed that it had strong superoxide anion radical-scavenging activity with the half-scavenging concentration (EC50) of about 16 mu g/mL. Therefore, the present study is the first time to demonstrate a high efficient method of isolating a novel antioxidant protein from the nematocyst of jellyfish S. meleagris. (C) 2012 Elsevier B.V. All rights reserved. |
| 关键词 | Jellyfish Stomolophus Meleagris Scavenging Superoxide Anion Antioxidant Isolation |
| 学科领域 | Biochemistry & Molecular Biology |
| DOI | 10.1016/j.ijbiomac.2012.05.015 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000307134200014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12232 |
| 专题 | 海洋生物技术研发中心 海洋环境工程技术研究发展中心 |
| 通讯作者 | Yu, HH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Rongfeng,Yu, Huahua,Xing, Ronge,et al. Isolation, identification and characterization of a novel antioxidant protein from the nematocyst of the jellyfish Stomolophus meleagris[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2012,51(3):274-278. |
| APA | Li, Rongfeng.,Yu, Huahua.,Xing, Ronge.,Liu, Song.,Qing, Yukun.,...&Yu, HH .(2012).Isolation, identification and characterization of a novel antioxidant protein from the nematocyst of the jellyfish Stomolophus meleagris.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,51(3),274-278. |
| MLA | Li, Rongfeng,et al."Isolation, identification and characterization of a novel antioxidant protein from the nematocyst of the jellyfish Stomolophus meleagris".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 51.3(2012):274-278. |
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