| Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris | |
Li, Rongfeng1,2; Yu, Huahua1 ; Xing, Ronge1; Liu, Song1; Qing, Yukun1,2; Li, Kecheng1,2; Li, Bing1,2; Meng, Xiangtao1,2; Cui, Jinhui1,2; Li, Pengcheng1; Yu, HH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
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| 2012-06-15 | |
| 发表期刊 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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| ISSN | 1570-0232 |
| 卷号 | 899页码:86-95 |
| 文章类型 | Article |
| 摘要 | The nematocyst proteins of jellyfish Stomolophus meleagris, a complicated mixture, contain many important bioactive molecules. In present study, to gain comprehensive insight into the protein component and search some novel bioactive molecules in the nematocyst proteins, shotgun proteomic analysis of the nematocyst proteins was carried out by nano liquid chromatography tandem mass spectrometry (nanoLC-MS/MS) for the first time. Digested peptides of the nematocyst proteins were analyzed by nanoLC-MS/MS and all MS/MS spectra were then automatically searched by the SEQUEST program. A total of 181 proteins had been identified, with the molecular weight ranging from 5268.06 to 843,487.57 and the pi from 4.49 to 11.39. Bioinformatic analysis was also applied to better understand the identified proteins. In the gene ontology (GO) annotation, all the identified proteins were classified into 13.9 and 7 groups according to biological process, cellular component and molecular function, respectively. Pathways analysis of the identified proteins was conducted with 33 corresponding pathways found. On the basis of pathways analysis, we also constructed the gene network to analyze the relationship of those genes each other, which contained enzyme-enzyme relation, protein-protein interaction and gene expression interaction. (C) 2012 Elsevier B.V. All rights reserved. |
| 关键词 | Jellyfish Stomolophus Meleagris Nematocyst Proteins Shotgun Nanolc-ms/ms Proteomic Analysis |
| 学科领域 | Biochemical Research Methods ; Chemistry, Analytical |
| DOI | 10.1016/j.jchromb.2012.05.006 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000306247300013 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12213 |
| 专题 | 海洋生物技术研发中心 海洋环境工程技术研究发展中心 |
| 通讯作者 | Yu, HH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Rongfeng,Yu, Huahua,Xing, Ronge,et al. Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,899:86-95. |
| APA | Li, Rongfeng.,Yu, Huahua.,Xing, Ronge.,Liu, Song.,Qing, Yukun.,...&Yu, HH .(2012).Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,899,86-95. |
| MLA | Li, Rongfeng,et al."Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 899(2012):86-95. |
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