| Two-step purification and in vitro characterization of a hemolysin from the venom of jellyfish Cyanea nozakii Kishinouye | |
Li, Rongfeng1,2; Yu, Huahua1 ; Feng, Jinhua1; Xing, Ronge1; Liu, Song1; Wang, Lin1,2; Qin, Yukun1,2; Li, Kecheng1,2; Li, Pengcheng1
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| 2011-07-01 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| ISSN | 0141-8130 |
| 卷号 | 49期号:1页码:14-19 |
| 文章类型 | Article |
| 摘要 | Hemolysin is one of the most hazardous components in the venom of Cyanea nozakii Kishinouye. Here we describe the purification and in vitro characterization of the hemolysin, which we named CnPH. The CnPH was isolated by anion-exchange and size-exclusion chromatography from the nematocyst venom. Two protein bands with molecular masses of 20 kDa, 60 kDa respectively were shown in the reducing SOS-PAGE analysis of the CnPH. And Approximately 5 mu g/mL of the CnPH resulted in 50% hemolysis of the erythrocyte suspension. The hemolytic activity of the CnPH was both temperature and pH dependent. Moreover, it was significantly inhibited in the presence of divalent metal cations, including Cu(2+), Mg(2+), Mn(2+), Zn(2+) and Ca(2+), but enhanced in the presence of EDTA. However, how CnPH performs its hemolytic activity is not yet clear, therefore the mechanism of the hemolytic activity of the CnPH is under research. (C) 2011 Elsevier B.V. All rights reserved.; Hemolysin is one of the most hazardous components in the venom of Cyanea nozakii Kishinouye. Here we describe the purification and in vitro characterization of the hemolysin, which we named CnPH. The CnPH was isolated by anion-exchange and size-exclusion chromatography from the nematocyst venom. Two protein bands with molecular masses of 20 kDa, 60 kDa respectively were shown in the reducing SOS-PAGE analysis of the CnPH. And Approximately 5 mu g/mL of the CnPH resulted in 50% hemolysis of the erythrocyte suspension. The hemolytic activity of the CnPH was both temperature and pH dependent. Moreover, it was significantly inhibited in the presence of divalent metal cations, including Cu(2+), Mg(2+), Mn(2+), Zn(2+) and Ca(2+), but enhanced in the presence of EDTA. However, how CnPH performs its hemolytic activity is not yet clear, therefore the mechanism of the hemolytic activity of the CnPH is under research. (C) 2011 Elsevier B.V. All rights reserved. |
| 关键词 | Jellyfish Cyanea Nozakii Kishinouye Hemolysis Venom |
| 学科领域 | Biochemistry & Molecular Biology |
| DOI | 10.1016/j.ijbiomac.2011.03.005 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291840400003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12057 |
| 专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 海洋环境工程技术研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Rongfeng,Yu, Huahua,Feng, Jinhua,et al. Two-step purification and in vitro characterization of a hemolysin from the venom of jellyfish Cyanea nozakii Kishinouye[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2011,49(1):14-19. |
| APA | Li, Rongfeng.,Yu, Huahua.,Feng, Jinhua.,Xing, Ronge.,Liu, Song.,...&Li, Pengcheng.(2011).Two-step purification and in vitro characterization of a hemolysin from the venom of jellyfish Cyanea nozakii Kishinouye.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,49(1),14-19. |
| MLA | Li, Rongfeng,et al."Two-step purification and in vitro characterization of a hemolysin from the venom of jellyfish Cyanea nozakii Kishinouye".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 49.1(2011):14-19. |
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