Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles

doi:10.1016/j.jchromb.2011.03.014

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	Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles
	Chen, Yingjie 1,2; Jiang, Peng 1; Liu, Shaofang 1,2; Zhao, Hui 1,2; Cui, Yulin 1,2; Qin, Song 1
	2011-04-15
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
卷号	879 期号:13-14 页码:993-997
文章类型	Article
摘要	Zinc-decorated silica-coated magnetic nanoparticles (ZnSiMNPs) were prepared by adsorbing zinc onto colloidal silica. These nanoparticles were used for the rapid purification of 6xHis-tagged recombinant phycobiliprotein. The surface changes in the magnetic nanoparticles after zinc adsorption were characterized by transmission electron microscopy. The adsorption of the 6xHis-tagged phycobiliprotein onto ZnSiMNPs in 10 mM PBS at 25 degrees C was found to follow the Langmuir isotherm. ZnSiMNPs could be used to extract 6xHis-tagged phycobiliprotein from lysate to single-band purity, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. No spectral variation was observed in the purified phycobiliprotein. Thus, ZnSiMNPs served as a useful tool for the magnetic separation and delivery of the 6xHis-tagged phycobiliprotein. (C) 2011 Elsevier B.V. All rights reserved.; Zinc-decorated silica-coated magnetic nanoparticles (ZnSiMNPs) were prepared by adsorbing zinc onto colloidal silica. These nanoparticles were used for the rapid purification of 6xHis-tagged recombinant phycobiliprotein. The surface changes in the magnetic nanoparticles after zinc adsorption were characterized by transmission electron microscopy. The adsorption of the 6xHis-tagged phycobiliprotein onto ZnSiMNPs in 10 mM PBS at 25 degrees C was found to follow the Langmuir isotherm. ZnSiMNPs could be used to extract 6xHis-tagged phycobiliprotein from lysate to single-band purity, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. No spectral variation was observed in the purified phycobiliprotein. Thus, ZnSiMNPs served as a useful tool for the magnetic separation and delivery of the 6xHis-tagged phycobiliprotein. (C) 2011 Elsevier B.V. All rights reserved.
关键词	6xhis Tag Phycobiliprotein Silica-coated Magnetic Nanoparticles (Simnps) Zn(Ii) Purification Adsorption Behavior
学科领域	Biochemistry & Molecular Biology ; Chemistry
DOI	10.1016/j.jchromb.2011.03.014
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000290005400025
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11925
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Chen, Yingjie,Jiang, Peng,Liu, Shaofang,et al. Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(13-14):993-997.
APA	Chen, Yingjie,Jiang, Peng,Liu, Shaofang,Zhao, Hui,Cui, Yulin,&Qin, Song.(2011).Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(13-14),993-997.
MLA	Chen, Yingjie,et al."Purification of 6xHis-tagged phycobiliprotein using zinc-decorated silica-coated magnetic nanoparticles".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.13-14(2011):993-997.

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