Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)

doi:10.1007/s00343-011-0295-0

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	Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)
	Wang Jinxia 1,2; Shao Kuishuang 3; Cheng Bin 4; Lu Qinqin 4; Zhou Baicheng 1
	2011-11-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	29 期号:6 页码:1260-1266
文章类型	Article
摘要	A filamentous clone of Polysiphonia urceolata was regenerated from segments cut from the fronds of gametophytes. Unlike wild thalli with short virgate branchlets, the clone was filamentous with few branches. Many transparent trichoblasts arose from pericentral cells during the induction culture, but these were seldom observed during normal growth. The trichoblasts were uniseriate, often colorless, and formed lobed rhizoids rapidly when they came into contact with solid substrates. In addition to morphological characteristics, the photosynthetic properties and growth conditions of the clone differed from those of the mother plant. Cross-gradient light and temperature culture experiments revealed that the most favorable conditions for culture of the filamentous clone were 22 degrees C and 95-120 mu E/(m(2).s) light intensity. The photosynthetic light saturation value for filaments was approx. 100 mu E/(m(2).s), which is far lower than that of wild thalli. These results could be used to develop techniques for mass cultures of P. urceolata in photobioreactors for production of seed stock or bioactive products.; A filamentous clone of Polysiphonia urceolata was regenerated from segments cut from the fronds of gametophytes. Unlike wild thalli with short virgate branchlets, the clone was filamentous with few branches. Many transparent trichoblasts arose from pericentral cells during the induction culture, but these were seldom observed during normal growth. The trichoblasts were uniseriate, often colorless, and formed lobed rhizoids rapidly when they came into contact with solid substrates. In addition to morphological characteristics, the photosynthetic properties and growth conditions of the clone differed from those of the mother plant. Cross-gradient light and temperature culture experiments revealed that the most favorable conditions for culture of the filamentous clone were 22 degrees C and 95-120 mu E/(m(2).s) light intensity. The photosynthetic light saturation value for filaments was approx. 100 mu E/(m(2).s), which is far lower than that of wild thalli. These results could be used to develop techniques for mass cultures of P. urceolata in photobioreactors for production of seed stock or bioactive products.
关键词	Polysiphonia Urceolata Rhodophyta Filament Culture
学科领域	Marine & Freshwater Biology ; Oceanography
DOI	10.1007/s00343-011-0295-0
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000299039400013
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11879
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Natl Marine Environm Monitoring Ctr, Dalian 116023, Peoples R China 4.Inst Oceanol & Marine Fisheries, Nantong 226007, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang Jinxia,Shao Kuishuang,Cheng Bin,et al. Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2011,29(6):1260-1266.
APA	Wang Jinxia,Shao Kuishuang,Cheng Bin,Lu Qinqin,&Zhou Baicheng.(2011).Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta).CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,29(6),1260-1266.
MLA	Wang Jinxia,et al."Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 29.6(2011):1260-1266.

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