Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection | |
Luo Zhan1,2; Zhang Jiquan1; Li Fuhua1; Zhang Xiaojun1; Liu Chengzhang1,2; Xiang Jianhai1
| |
| 2011-09-01 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| ISSN | 0254-4059 |
| 卷号 | 29期号:5页码:942-951 |
| 文章类型 | Article |
| 摘要 | Acting as one of the pattern recognition receptors (PRRs), C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In this work, a novel C-type lectin gene (named LvLec1) was cloned from the shrimp Litopenaeus vannamei. The ORF of LvLec1 is 510 bp, encoding 169 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain (CRD) at the C-terminal. LvLec1 was mainly expressed in the hepatopancreas. Real-time PCR analysis indicated that the level of LvLec1 transcripts significantly changed in the hepatopancreas after the shrimp were artificially challenged with LPS, Micrococcus lysodeikticus and white spot syndrome virus (WSSV). RNAi-based silencing of LvLec1 resulted in increases in mortality when the shrimp were challenged with WSSV, and the median lethal time was reduced compared with controls. Although there was no characteristic "EPN" (Glu-Pro-Ser) or "QPD" (Gln-Pro-Asp) motif, the recombinant LvLec1, expressed in Escherichia coli BL21 (DE3), could also agglutinate M. lysodeikticus and Vibrio anguillarum. The agglutinating activities were calcium-dependent and could be inhibited by D-mannose, D-glucose, D-galactose and N-Acetyl-D-mannose. These results suggest that LvLec1 might be involved in the immune response against WSSV and bacterial infections and contribute to non-self recognition as a pattern recognition receptor in the innate immune system of the shrimp L. vannamei.; Acting as one of the pattern recognition receptors (PRRs), C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In this work, a novel C-type lectin gene (named LvLec1) was cloned from the shrimp Litopenaeus vannamei. The ORF of LvLec1 is 510 bp, encoding 169 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain (CRD) at the C-terminal. LvLec1 was mainly expressed in the hepatopancreas. Real-time PCR analysis indicated that the level of LvLec1 transcripts significantly changed in the hepatopancreas after the shrimp were artificially challenged with LPS, Micrococcus lysodeikticus and white spot syndrome virus (WSSV). RNAi-based silencing of LvLec1 resulted in increases in mortality when the shrimp were challenged with WSSV, and the median lethal time was reduced compared with controls. Although there was no characteristic "EPN" (Glu-Pro-Ser) or "QPD" (Gln-Pro-Asp) motif, the recombinant LvLec1, expressed in Escherichia coli BL21 (DE3), could also agglutinate M. lysodeikticus and Vibrio anguillarum. The agglutinating activities were calcium-dependent and could be inhibited by D-mannose, D-glucose, D-galactose and N-Acetyl-D-mannose. These results suggest that LvLec1 might be involved in the immune response against WSSV and bacterial infections and contribute to non-self recognition as a pattern recognition receptor in the innate immune system of the shrimp L. vannamei. |
| 关键词 | Litopenaeus Vannamei Innate Immune Pattern Recognition Receptor C-type Lectin Rna Interference (Rnai) Recombinant Expression |
| 学科领域 | Marine & Freshwater Biology ; Oceanography |
| DOI | 10.1007/s00343-011-0249-6 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000293632500004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/11875 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Luo Zhan,Zhang Jiquan,Li Fuhua,et al. Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2011,29(5):942-951. |
| APA | Luo Zhan,Zhang Jiquan,Li Fuhua,Zhang Xiaojun,Liu Chengzhang,&Xiang Jianhai.(2011).Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,29(5),942-951. |
| MLA | Luo Zhan,et al."Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 29.5(2011):942-951. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Identification of a （410KB） | 限制开放 | -- | 浏览 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论