Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus

doi:10.1016/j.fsi.2010.10.003

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	Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus
	Zhang, Min; Xiao, Zhi-zhong; Sun, Li
	2011
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	30 期号:1 页码:209-214
文章类型	Article
摘要	Tissue factor pathway inhibitor 2 (TFPI-2) is a structural homologue of TFPI, a potent inhibitor of tissue factor (TF)-mediated coagulation. Although TFPI-2 has been identified at sequence level in several fish species, no study on piscine TFPI-2 has been documented. In this report, we identified and analyzed a TFPI-2 homologue, SoTFPI2, from red drum Sciaenops ocellatus. The open reading frame of SoTFPI2 is 681 bp, which encodes a 226-residue protein that shares 59.2-82.3% overall sequence identities with known fish TFPI-2. SoTFPI2 possesses three tandem Kunitz domains and is negatively charged at the N-terminus and positively charged at the C-terminus. Expression of SoTFPI2 was detected, in increasing order, in spleen, muscle, gill, brain, liver, kidney, blood, and heart. Bacterial challenge and lipopolysaccharide treatment significantly upregulated SoTFPI2 expression in kidney in time-dependent manners. Recombinant SoTFPI2 purified from Escherichia colt inhibits the proteolytic activity of trypsin and exhibits bactericidal effect on a fish pathogen. Take together, these results indicate that SoTFPI2 is a biologically active serine protease inhibitor with antibacterial property and is likely to play a role in anti-bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.; Tissue factor pathway inhibitor 2 (TFPI-2) is a structural homologue of TFPI, a potent inhibitor of tissue factor (TF)-mediated coagulation. Although TFPI-2 has been identified at sequence level in several fish species, no study on piscine TFPI-2 has been documented. In this report, we identified and analyzed a TFPI-2 homologue, SoTFPI2, from red drum Sciaenops ocellatus. The open reading frame of SoTFPI2 is 681 bp, which encodes a 226-residue protein that shares 59.2-82.3% overall sequence identities with known fish TFPI-2. SoTFPI2 possesses three tandem Kunitz domains and is negatively charged at the N-terminus and positively charged at the C-terminus. Expression of SoTFPI2 was detected, in increasing order, in spleen, muscle, gill, brain, liver, kidney, blood, and heart. Bacterial challenge and lipopolysaccharide treatment significantly upregulated SoTFPI2 expression in kidney in time-dependent manners. Recombinant SoTFPI2 purified from Escherichia colt inhibits the proteolytic activity of trypsin and exhibits bactericidal effect on a fish pathogen. Take together, these results indicate that SoTFPI2 is a biologically active serine protease inhibitor with antibacterial property and is likely to play a role in anti-bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved.
关键词	Tissue Factor Pathway Inhibitor 2 Sciaenops Ocellatus Bactericidal Activity
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2010.10.003
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000286905800026
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11869
专题	实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Min,Xiao, Zhi-zhong,Sun, Li. Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(1):209-214.
APA	Zhang, Min,Xiao, Zhi-zhong,&Sun, Li.(2011).Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus.FISH & SHELLFISH IMMUNOLOGY,30(1),209-214.
MLA	Zhang, Min,et al."Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus".FISH & SHELLFISH IMMUNOLOGY 30.1(2011):209-214.

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