Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| An interleukin-2 enhancer binding factor 2 homolog involved in immune response from Chinese mitten crab Eriocheir sinensis | |
Yang, Jialong1,2; Wang, Lingling1; Huang, Mengmeng1,2 ; Wang, Leilei1,2; Gai, Yunchao1; Qiu, Limei1; Zhang, Huan1,2; Song, Linsheng1
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| 2011-06-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 30期号:6页码:1303-1309 |
| 文章类型 | Article |
| 摘要 | As a transcription factor, Interleukin-2 enhancer binding factor 2 (ILF2) regulates IL-2 gene at level of transcription, splicing and translation in vertebrates and plays significant roles in immune system. In this study, an ILF2 homolog was identified from Chinese mitten crab Eriocheir sinensis (designated as EsILF) by expressed sequence tag (EST) analysis. The full-length cDNA of EsILF was of 2159 bp, containing a 5' untranslated region (UTR) of 90 bp, a 3' UTR of 866 bp with a poly (A) tail, and an open reading frame (ORF) of 1203 bp encoding a polypeptide of 400 amino acids with the predicted molecular weight of 44.3 kDa, which shared 59.6-64.5% identities with vertebrate ILF2. There were a conserved N-terminal RGG-rich single-stranded RNA-binding domain and a DZF zinc-finger nucleic acid binding domain in the primary structure, strongly suggesting that EsILF was a homolog of vertebrate ILF2. The mRNA of EsILF was constitutively expressed in all tested tissues of untreated crabs, including hepatopancreas, gill, gonad, muscle, heart and hemocytes, with highest expression in muscle and relative lower levels in hemocytes and gonad. The mRNA expression of EsILF in hemocytes was regulated differently after the crabs were stimulated by bacteria Listonella anguillarum and fungi Pichia pastoris GS115. The expression level was significantly (P < 0.05) down-regulated to 0.35- and 0.29-fold compared with blank group at 6 h and 12 h after the stimulation of L. anguillarum, while P pastoris significantly (P < 0.05) up-regulated the expression level to 3.2-fold compared with the blank group at 6 h post treatment. The results indicated that EsILF was involved in the immune response of crab toward both L. anguillarum and P. pastoris. (C) 2011 Elsevier Ltd. All rights reserved.; As a transcription factor, Interleukin-2 enhancer binding factor 2 (ILF2) regulates IL-2 gene at level of transcription, splicing and translation in vertebrates and plays significant roles in immune system. In this study, an ILF2 homolog was identified from Chinese mitten crab Eriocheir sinensis (designated as EsILF) by expressed sequence tag (EST) analysis. The full-length cDNA of EsILF was of 2159 bp, containing a 5' untranslated region (UTR) of 90 bp, a 3' UTR of 866 bp with a poly (A) tail, and an open reading frame (ORF) of 1203 bp encoding a polypeptide of 400 amino acids with the predicted molecular weight of 44.3 kDa, which shared 59.6-64.5% identities with vertebrate ILF2. There were a conserved N-terminal RGG-rich single-stranded RNA-binding domain and a DZF zinc-finger nucleic acid binding domain in the primary structure, strongly suggesting that EsILF was a homolog of vertebrate ILF2. The mRNA of EsILF was constitutively expressed in all tested tissues of untreated crabs, including hepatopancreas, gill, gonad, muscle, heart and hemocytes, with highest expression in muscle and relative lower levels in hemocytes and gonad. The mRNA expression of EsILF in hemocytes was regulated differently after the crabs were stimulated by bacteria Listonella anguillarum and fungi Pichia pastoris GS115. The expression level was significantly (P < 0.05) down-regulated to 0.35- and 0.29-fold compared with blank group at 6 h and 12 h after the stimulation of L. anguillarum, while P pastoris significantly (P < 0.05) up-regulated the expression level to 3.2-fold compared with the blank group at 6 h post treatment. The results indicated that EsILF was involved in the immune response of crab toward both L. anguillarum and P. pastoris. (C) 2011 Elsevier Ltd. All rights reserved. |
| 关键词 | Il-2 System Ilf2 Transcription Factor Eriocheir Sinensis Immune Response |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2011.03.014 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291838400011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/11779 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Yang, Jialong,Wang, Lingling,Huang, Mengmeng,et al. An interleukin-2 enhancer binding factor 2 homolog involved in immune response from Chinese mitten crab Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(6):1303-1309. |
| APA | Yang, Jialong.,Wang, Lingling.,Huang, Mengmeng.,Wang, Leilei.,Gai, Yunchao.,...&Song, Linsheng.(2011).An interleukin-2 enhancer binding factor 2 homolog involved in immune response from Chinese mitten crab Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,30(6),1303-1309. |
| MLA | Yang, Jialong,et al."An interleukin-2 enhancer binding factor 2 homolog involved in immune response from Chinese mitten crab Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 30.6(2011):1303-1309. |
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