An inflammatory CC chemokine of Cynoglossus semilaevis is involved in immune defense against bacterial infection

doi:10.1016/j.fsi.2011.06.017

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	An inflammatory CC chemokine of Cynoglossus semilaevis is involved in immune defense against bacterial infection
	Li, Yong-xin 1,2; Sun, Jin-sheng 2; Sun, Li 1
	2011-09-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	31 期号:3 页码:446-452
文章类型	Article
摘要	Chemokines are a family of small cytokines that regulate leukocyte migration. Based on the arrangement of the first two cysteine residues, chemokines are classified into four groups called CXC(alpha), CC(beta), C, and CX(3)C. In this study, we identified a CC chemokine, C5CCK1, from half-smooth tongue sole (Cynoglossus semilaevis) and analyzed its biological activity. The deduced amino acid sequence of CsCCK1 contains 111 amino acid residues and is phylogenetically belonging to the CCL19/21/25 group of CC chemokines. CsCCK1 possesses a DCCL motif that is highly conserved among CC chemokines. Quantitative real time RT-PCR analysis showed that the expression of CsCCK1 was relatively abundant in immune organs under normal physiological conditions and was upregulated by experimental infection of a bacterial pathogen. Purified recombinant CsCCK1 (rC5CCK1) induced chemotaxis in peripheral blood leukocytes (PBL) of both tongue sole and turbot (Scophthalmus maximus) in a dose-dependent manner. Mutation of the CC residues in the DCCL motif by serine substitution completely abolished the biological activity of rC5CCK1. When rC5CCK1, but not the mutant protein, was added to the cell culture of PBL, it enhanced cellular resistance against intracellular bacterial infection. Taken together, these results indicate that C5CCK1 is a functional CC chemokine whose biological activity depends on the DCCL motif and that C5CCK1 plays a role in host immune defense against bacterial infection. (C) 2011 Elsevier Ltd. All rights reserved.; Chemokines are a family of small cytokines that regulate leukocyte migration. Based on the arrangement of the first two cysteine residues, chemokines are classified into four groups called CXC(alpha), CC(beta), C, and CX(3)C. In this study, we identified a CC chemokine, C5CCK1, from half-smooth tongue sole (Cynoglossus semilaevis) and analyzed its biological activity. The deduced amino acid sequence of CsCCK1 contains 111 amino acid residues and is phylogenetically belonging to the CCL19/21/25 group of CC chemokines. CsCCK1 possesses a DCCL motif that is highly conserved among CC chemokines. Quantitative real time RT-PCR analysis showed that the expression of CsCCK1 was relatively abundant in immune organs under normal physiological conditions and was upregulated by experimental infection of a bacterial pathogen. Purified recombinant CsCCK1 (rC5CCK1) induced chemotaxis in peripheral blood leukocytes (PBL) of both tongue sole and turbot (Scophthalmus maximus) in a dose-dependent manner. Mutation of the CC residues in the DCCL motif by serine substitution completely abolished the biological activity of rC5CCK1. When rC5CCK1, but not the mutant protein, was added to the cell culture of PBL, it enhanced cellular resistance against intracellular bacterial infection. Taken together, these results indicate that C5CCK1 is a functional CC chemokine whose biological activity depends on the DCCL motif and that C5CCK1 plays a role in host immune defense against bacterial infection. (C) 2011 Elsevier Ltd. All rights reserved.
关键词	Cc Chemokine Cynoglossus Semilaevis Chemotaxis
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2011.06.017
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000294150800011
引用统计	被引频次：45[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11777
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Tianjin Normal Univ, Coll Life Sci, Tianjin 300387, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Li, Yong-xin,Sun, Jin-sheng,Sun, Li. An inflammatory CC chemokine of Cynoglossus semilaevis is involved in immune defense against bacterial infection[J]. FISH & SHELLFISH IMMUNOLOGY,2011,31(3):446-452.
APA	Li, Yong-xin,Sun, Jin-sheng,&Sun, Li.(2011).An inflammatory CC chemokine of Cynoglossus semilaevis is involved in immune defense against bacterial infection.FISH & SHELLFISH IMMUNOLOGY,31(3),446-452.
MLA	Li, Yong-xin,et al."An inflammatory CC chemokine of Cynoglossus semilaevis is involved in immune defense against bacterial infection".FISH & SHELLFISH IMMUNOLOGY 31.3(2011):446-452.

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