A novel type III crustin (CrusEs2) identified from Chinese mitten crab Eriocheir sinensis

doi:10.1016/j.fsi.2011.04.013

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	A novel type III crustin (CrusEs2) identified from Chinese mitten crab Eriocheir sinensis
	Mu, Changkao 1,2; Zheng, Peilin 1; Zhao, Jianmin 1; Wang, Lingling 1; Qiu, Limei 1; Zhang, Huan 1; Gai, Yunchao 1; Song, Linsheng 1
	2011-07-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	31 期号:1 页码:142-147
文章类型	Article
摘要	Antimicrobial peptides are important effectors in the host innate immune response against microbial invasion. In the present study, the cDNA encoding a crustin (designated CrusEs2) was cloned from Chinese mitten crab Eriocheir sinensis by using EST analysis and rapid amplification of cDNA ends (RACE) approach. The full-length cDNA of CrusEs2 was of 1237 bp, containing a 5' untranslated region (UTR) of 12 bp, a 3' UTR of 886 bp with a poly (A) tail, and an open reading frame (ORF) of 339 bp encoding a polypeptide of 112 amino acids with a signal peptide of 19 amino acids. The CrusEs2 contained a typical WAP domain, but lacked the Gly-rich domain of the type II crustin and the Cys-rich region present in both type I and type II crustin, suggesting that CrusEs2 should be classified as a type III crustin. The mRNA transcripts of CrusEs2 could be detected in haemocytes and gill, and its expression level in haemocytes was up-regulated after Listonella anguillarum challenge, while decreased after Micrococcus luteus challenge. The mature peptide coding region of CrusEs2 was cloned into pET-21a (+) and expressed in Escherichia coli. The purified recombinant CrusEs2 inhibited the growth of Gram-positive bacteria at MIC of 0.093-0.37 mu M. The results indicated that CrusEs2 was involved in immune response of E. sinensis against bacterial challenge. (C) 2011 Elsevier Ltd. All rights reserved.; Antimicrobial peptides are important effectors in the host innate immune response against microbial invasion. In the present study, the cDNA encoding a crustin (designated CrusEs2) was cloned from Chinese mitten crab Eriocheir sinensis by using EST analysis and rapid amplification of cDNA ends (RACE) approach. The full-length cDNA of CrusEs2 was of 1237 bp, containing a 5' untranslated region (UTR) of 12 bp, a 3' UTR of 886 bp with a poly (A) tail, and an open reading frame (ORF) of 339 bp encoding a polypeptide of 112 amino acids with a signal peptide of 19 amino acids. The CrusEs2 contained a typical WAP domain, but lacked the Gly-rich domain of the type II crustin and the Cys-rich region present in both type I and type II crustin, suggesting that CrusEs2 should be classified as a type III crustin. The mRNA transcripts of CrusEs2 could be detected in haemocytes and gill, and its expression level in haemocytes was up-regulated after Listonella anguillarum challenge, while decreased after Micrococcus luteus challenge. The mature peptide coding region of CrusEs2 was cloned into pET-21a (+) and expressed in Escherichia coli. The purified recombinant CrusEs2 inhibited the growth of Gram-positive bacteria at MIC of 0.093-0.37 mu M. The results indicated that CrusEs2 was involved in immune response of E. sinensis against bacterial challenge. (C) 2011 Elsevier Ltd. All rights reserved.
关键词	Eriocheir Sinensis Crustin Bacteria Challenge Antibacterial Activity Minimal Inhibitory Concentration (Mic)
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2011.04.013
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000292407900017
引用统计	被引频次：61[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11759
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Mu, Changkao,Zheng, Peilin,Zhao, Jianmin,et al. A novel type III crustin (CrusEs2) identified from Chinese mitten crab Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2011,31(1):142-147.
APA	Mu, Changkao.,Zheng, Peilin.,Zhao, Jianmin.,Wang, Lingling.,Qiu, Limei.,...&Song, Linsheng.(2011).A novel type III crustin (CrusEs2) identified from Chinese mitten crab Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,31(1),142-147.
MLA	Mu, Changkao,et al."A novel type III crustin (CrusEs2) identified from Chinese mitten crab Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 31.1(2011):142-147.

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