A Dopa Decarboxylase Modulating the Immune Response of Scallop Chlamys farreri

doi:10.1371/journal.pone.0018596

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	A Dopa Decarboxylase Modulating the Immune Response of Scallop Chlamys farreri
	Zhou, Zhi 1,2; Yang, Jialong 1,2; Wang, Lingling 1; Zhang, Huan 1; Gao, Yang1,2 ; Shi, Xiaowei 1,2; Wang, Mengqiang1 ; Kong, Pengfei 1,2; Qiu, Limei 1; Song, Linsheng 1
	2011-04-13
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	6 期号:4 页码:e18596
文章类型	Article
摘要	Background: Dopa decarboxylase (DDC) is a pyridoxal 5-phosphate (PLP)-dependent enzyme that catalyzes the decarboxylation of L-Dopa to dopamine, and involved in complex neuroendocrine-immune regulatory network. The function for DDC in the immunomodulation remains unclear in invertebrate.; Background: Dopa decarboxylase (DDC) is a pyridoxal 5-phosphate (PLP)-dependent enzyme that catalyzes the decarboxylation of L-Dopa to dopamine, and involved in complex neuroendocrine-immune regulatory network. The function for DDC in the immunomodulation remains unclear in invertebrate. Methodology: The full-length cDNA encoding DDC (designated CfDDC) was cloned from mollusc scallop Chlamys farreri. It contained an open reading frame encoding a polypeptide of 560 amino acids. The CfDDC mRNA transcripts could be detected in all the tested tissues, including the immune tissues haemocytes and hepatopancreas. After scallops were treated with LPS stimulation, the mRNA expression level of CfDDC in haemocytes increased significantly (5.5-fold, P<0.05) at 3 h and reached the peak at 12 h (9.8-fold, P<0.05), and then recovered to the baseline level. The recombinant protein of CfDDC (rCfDDC) was expressed in Escherichia coli BL21 (DE3)-Transetta, and 1 mg rCfDDC could catalyze the production of 1.651 +/- 0.22 ng dopamine within 1 h in vitro. When the haemocytes were incubated with rCfDDC-coated agarose beads, the haemocyte encapsulation to the beads was increased significantly from 70% at 6 h to 93% at 24 h in vitro in comparison with that in the control (23% at 6 h to 25% at 24 h), and the increased haemocyte encapsulation was repressed by the addition of rCfDDC antibody (which is acquired via immunization 6-week old rats with rCfDDC). After the injection of DDC inhibitor methyldopa, the ROS level in haemocytes of scallops was decreased significantly to 0.41-fold (P<0.05) of blank group at 12 h and 0.47-fold (P<0.05) at 24 h, respectively. Conclusions: These results collectively suggested that CfDDC, as a homologue of DDC in scallop, modulated the immune responses such as haemocytes encapsulation as well as the ROS level through its catalytic activity, functioning as an indispensable immunomodulating enzyme in the neuroendocrine-immune regulatory network of mollusc.
学科领域	Life Sciences & Biomedicine - Other Topics
DOI	10.1371/journal.pone.0018596
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000289458800021
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11741
专题	实验海洋生物学重点实验室海洋环境腐蚀与生物污损重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Zhi,Yang, Jialong,Wang, Lingling,et al. A Dopa Decarboxylase Modulating the Immune Response of Scallop Chlamys farreri[J]. PLOS ONE,2011,6(4):e18596.
APA	Zhou, Zhi.,Yang, Jialong.,Wang, Lingling.,Zhang, Huan.,Gao, Yang.,...&Song, Linsheng.(2011).A Dopa Decarboxylase Modulating the Immune Response of Scallop Chlamys farreri.PLOS ONE,6(4),e18596.
MLA	Zhou, Zhi,et al."A Dopa Decarboxylase Modulating the Immune Response of Scallop Chlamys farreri".PLOS ONE 6.4(2011):e18596.

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