A bivalent Vibrio harveyi DNA vaccine induces strong protection in Japanese flounder (Paralichthys olivaceus)

doi:10.1016/j.vaccine.2011.04.021

IOCAS-IR > 实验海洋生物学重点实验室

	A bivalent Vibrio harveyi DNA vaccine induces strong protection in Japanese flounder (Paralichthys olivaceus)
	Hu, Yong-Hua; Sun, Li
	2011-06-10
发表期刊	VACCINE
ISSN	0264-410X
卷号	29 期号:26 页码:4328-4333
文章类型	Article
摘要	Vibrio harveyi is a severe aquaculture pathogen that can infect a large number of fish species. In previous studies, we have identified two protective immunogens, DegQ and Vhp1, from pathogenic V. harveyi strains. In this study, we examined the potentials of DegQ and Vhp1 as monovalent and bivalent DNA vaccines in a Japanese flounder (Paralichthys olivaceus) model. For this purpose, the DNA vaccine plasmids pDegQ pVhp1, and pDV were constructed. pDegQ and pVhp1 express, respectively, degQ and vhp1, while pDV expresses degQ and v hp 1 as a bicistronic mRNA transcript (degQ-vhp1). Following vaccination, all vaccine plasmids were detected in muscle, spleen, and kidney at 7 and 14 days post-vaccination. At the same time points and in the same tissues, degQ, vhp1, and degQ-vhp1 mRNA transcripts were detected in, respectively, pDegQ-, pVhp1-, and pDV-vaccinated fish. Production of DegQ and Vhp1 proteins was found in the muscle tissues of pDegQ- and pVhp1-vaccinated fish respectively, while both DegQ and Vhp1 proteins were detected in pDV-vaccinated fish. Compared to control fish, fish vaccinated with pDegQ and pVhp1 exhibited, respectively, 64.1% and 56.5% relative percent survival rates following lethal V. harveyi challenge, while fish vaccinated with pDV exhibited a survival rate that is more than 20% higher than those induced by pDegQ and pVhp1. Furthermore, in addition to V. harveyi, pDV also elicited dramatic cross protection against Vibrio parahaemolyticus. Immunological analysis showed that all vaccine plasmids induced the production of specific serum antibodies and the expression of a wide range of immune genes; however, the induction folds effected by pDV were in general higher than those effected by pDegQ and pVhp1. These results indicate that DNA vaccines, such as that represented by pDV, is a good vaccination option to combat V. harveyi-related vibriosis. (C) 2011 Elsevier Ltd. All rights reserved.; Vibrio harveyi is a severe aquaculture pathogen that can infect a large number of fish species. In previous studies, we have identified two protective immunogens, DegQ and Vhp1, from pathogenic V. harveyi strains. In this study, we examined the potentials of DegQ and Vhp1 as monovalent and bivalent DNA vaccines in a Japanese flounder (Paralichthys olivaceus) model. For this purpose, the DNA vaccine plasmids pDegQ pVhp1, and pDV were constructed. pDegQ and pVhp1 express, respectively, degQ and vhp1, while pDV expresses degQ and v hp 1 as a bicistronic mRNA transcript (degQ-vhp1). Following vaccination, all vaccine plasmids were detected in muscle, spleen, and kidney at 7 and 14 days post-vaccination. At the same time points and in the same tissues, degQ, vhp1, and degQ-vhp1 mRNA transcripts were detected in, respectively, pDegQ-, pVhp1-, and pDV-vaccinated fish. Production of DegQ and Vhp1 proteins was found in the muscle tissues of pDegQ- and pVhp1-vaccinated fish respectively, while both DegQ and Vhp1 proteins were detected in pDV-vaccinated fish. Compared to control fish, fish vaccinated with pDegQ and pVhp1 exhibited, respectively, 64.1% and 56.5% relative percent survival rates following lethal V. harveyi challenge, while fish vaccinated with pDV exhibited a survival rate that is more than 20% higher than those induced by pDegQ and pVhp1. Furthermore, in addition to V. harveyi, pDV also elicited dramatic cross protection against Vibrio parahaemolyticus. Immunological analysis showed that all vaccine plasmids induced the production of specific serum antibodies and the expression of a wide range of immune genes; however, the induction folds effected by pDV were in general higher than those effected by pDegQ and pVhp1. These results indicate that DNA vaccines, such as that represented by pDV, is a good vaccination option to combat V. harveyi-related vibriosis. (C) 2011 Elsevier Ltd. All rights reserved.
关键词	Dna Vaccine Vibrio Harveyi Cross Protection Immune Gene
学科领域	Immunology ; Research & Experimental Medicine
DOI	10.1016/j.vaccine.2011.04.021
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000292353600006
引用统计	被引频次：34[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11739
专题	实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Hu, Yong-Hua,Sun, Li. A bivalent Vibrio harveyi DNA vaccine induces strong protection in Japanese flounder (Paralichthys olivaceus)[J]. VACCINE,2011,29(26):4328-4333.
APA	Hu, Yong-Hua,&Sun, Li.(2011).A bivalent Vibrio harveyi DNA vaccine induces strong protection in Japanese flounder (Paralichthys olivaceus).VACCINE,29(26),4328-4333.
MLA	Hu, Yong-Hua,et al."A bivalent Vibrio harveyi DNA vaccine induces strong protection in Japanese flounder (Paralichthys olivaceus)".VACCINE 29.26(2011):4328-4333.

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