The intestinal microbial diversity in Chinese shrimp (Fenneropenaeus chinensis) as determined by PCR-DGGE and clone library analyses

doi:10.1016/j.aquaculture.2011.04.008

	The intestinal microbial diversity in Chinese shrimp (Fenneropenaeus chinensis) as determined by PCR-DGGE and clone library analyses
	Liu, Huaide 1; Wang, Lei 2; Liu, Mei 2; Wang, Baojie 2; Jiang, Keyong 2; Ma, Shaosai 1; Li, Qiufen 1
	2011-07-04
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	317 期号:1-4 页码:32-36
文章类型	Article
摘要	In this study, the intestinal microbiota of Chinese shrimp (Fenneropenaeus chinensis) was examined by molecular analysis of the 16S rDNA. The aims of the study were to evaluate the use of molecular-based techniques, Polymerase Chain Reaction/Denaturing Gradient Gel Electrophoresis (PCR-DGGE) and clone library analyses, and compare the results obtained by the two methods. Total DNA was extracted from the intestine of Chinese shrimp. Then population fingerprints of the predominant microbiota were generated by DGGE analysis of universal V3 16S rDNA PCR amplicons, and 22 distinct bands from DGGE were sequenced. In addition, bacterial 16S rRNA gene was amplified with universal bacterial primers 27F and 1387R. Amplicons were ligated into cloning vectors and near-full-length 16S rRNA gene inserts were analyzed. A clone library was constructed and 72 clones were sequenced. The results suggested that PCR DGGE and clone library analysis were effective techniques for monitoring and analyzing the intestinal microbial diversity of Chinese shrimp. The two different molecular biological methods gave similar results, but clone library analysis was more representative of the community to some extent. According to the two methods, Vibrio sp. was the predominant bacterial population in the intestine of Chinese shrimp. (C) 2011 Elsevier B.V. All rights reserved.; In this study, the intestinal microbiota of Chinese shrimp (Fenneropenaeus chinensis) was examined by molecular analysis of the 16S rDNA. The aims of the study were to evaluate the use of molecular-based techniques, Polymerase Chain Reaction/Denaturing Gradient Gel Electrophoresis (PCR-DGGE) and clone library analyses, and compare the results obtained by the two methods. Total DNA was extracted from the intestine of Chinese shrimp. Then population fingerprints of the predominant microbiota were generated by DGGE analysis of universal V3 16S rDNA PCR amplicons, and 22 distinct bands from DGGE were sequenced. In addition, bacterial 16S rRNA gene was amplified with universal bacterial primers 27F and 1387R. Amplicons were ligated into cloning vectors and near-full-length 16S rRNA gene inserts were analyzed. A clone library was constructed and 72 clones were sequenced. The results suggested that PCR DGGE and clone library analysis were effective techniques for monitoring and analyzing the intestinal microbial diversity of Chinese shrimp. The two different molecular biological methods gave similar results, but clone library analysis was more representative of the community to some extent. According to the two methods, Vibrio sp. was the predominant bacterial population in the intestine of Chinese shrimp. (C) 2011 Elsevier B.V. All rights reserved.
关键词	Intestinal Microbial Diversity Pcr-dgge 16s Rdna Clone Library Chinese Shrimp
学科领域	Fisheries ; Marine & Freshwater Biology
DOI	10.1016/j.aquaculture.2011.04.008
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000291908000005
引用统计	被引频次：85[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11717
专题	海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Liu, Huaide,Wang, Lei,Liu, Mei,et al. The intestinal microbial diversity in Chinese shrimp (Fenneropenaeus chinensis) as determined by PCR-DGGE and clone library analyses[J]. AQUACULTURE,2011,317(1-4):32-36.
APA	Liu, Huaide.,Wang, Lei.,Liu, Mei.,Wang, Baojie.,Jiang, Keyong.,...&Li, Qiufen.(2011).The intestinal microbial diversity in Chinese shrimp (Fenneropenaeus chinensis) as determined by PCR-DGGE and clone library analyses.AQUACULTURE,317(1-4),32-36.
MLA	Liu, Huaide,et al."The intestinal microbial diversity in Chinese shrimp (Fenneropenaeus chinensis) as determined by PCR-DGGE and clone library analyses".AQUACULTURE 317.1-4(2011):32-36.

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