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| Responses of the growth and biochemical composition of Prorocentrum donghaiense to different nitrogen and phosphorus concentrations | |
Lai, Junxiang1,2; Yu, Zhiming1 ; Song, Xiuxian1; Cao, Xihua1; Han, Xiaotian1
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| 2011-08-31 | |
| 发表期刊 | JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
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| ISSN | 0022-0981 |
| 卷号 | 405期号:1-2页码:6-17 |
| 文章类型 | Article |
| 摘要 | Responses of growth, cellular total protein, carbohydrates and pigment composition in batch cultures of the marine dinoflagellate, Prorocentrum donghaiense, isolated from the Changjiang Estuary were studied in relation to concentrations of nitrate (0-256 mu M) and phosphate (0-16 mu M). The results showed that growth and biochemical composition of P. donghaiense were affected by changes in concentrations of N and P. Results showed luxury P consumption in all treatments. Algal division rates were strongly dependent on nitrogen and phosphorus concentrations. An increase in the initial N-added concentrations of the media led to an increase in the cellular total protein, ranging from 0.931 to 0.575 ng/cell during stationary phase. P concentration did not have an obvious effect on protein. Cellular carbohydrates significantly accumulated in both N-limited and P-limited conditions. There was a significant negative correlation between cellular carbohydrates and the specific growth rate. Nutrient availability influenced pigment synthesis. Cellular pigment contents in N-O and P-O treatments were significantly lower than that for high nutrient treatments during stationary phase. In addition, pigment ratios changes with different growth phases and nutrient status. Relationship between these biochemical parameters and initial nutrient concentrations showed the potential for specific ratios, including cellular protein/carbohydrate, cellular beta-carotene/Chlorophyll a, cellular carbohydrate/cellular total P and protein/cellular total P. to be used as indicators of physiological status of P. donghaiense. (C) 2011 Elsevier B.V. All rights reserved.; Responses of growth, cellular total protein, carbohydrates and pigment composition in batch cultures of the marine dinoflagellate, Prorocentrum donghaiense, isolated from the Changjiang Estuary were studied in relation to concentrations of nitrate (0-256 mu M) and phosphate (0-16 mu M). The results showed that growth and biochemical composition of P. donghaiense were affected by changes in concentrations of N and P. Results showed luxury P consumption in all treatments. Algal division rates were strongly dependent on nitrogen and phosphorus concentrations. An increase in the initial N-added concentrations of the media led to an increase in the cellular total protein, ranging from 0.931 to 0.575 ng/cell during stationary phase. P concentration did not have an obvious effect on protein. Cellular carbohydrates significantly accumulated in both N-limited and P-limited conditions. There was a significant negative correlation between cellular carbohydrates and the specific growth rate. Nutrient availability influenced pigment synthesis. Cellular pigment contents in N-O and P-O treatments were significantly lower than that for high nutrient treatments during stationary phase. In addition, pigment ratios changes with different growth phases and nutrient status. Relationship between these biochemical parameters and initial nutrient concentrations showed the potential for specific ratios, including cellular protein/carbohydrate, cellular beta-carotene/Chlorophyll a, cellular carbohydrate/cellular total P and protein/cellular total P. to be used as indicators of physiological status of P. donghaiense. (C) 2011 Elsevier B.V. All rights reserved. |
| 关键词 | Carbohydrate Nutrient Limitation Pigments Prorocentrum Donghaiense Protein |
| 学科领域 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| DOI | 10.1016/j.jembe.2011.05.010 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000293047800002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/11699 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Lai, Junxiang,Yu, Zhiming,Song, Xiuxian,et al. Responses of the growth and biochemical composition of Prorocentrum donghaiense to different nitrogen and phosphorus concentrations[J]. JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY,2011,405(1-2):6-17. |
| APA | Lai, Junxiang,Yu, Zhiming,Song, Xiuxian,Cao, Xihua,&Han, Xiaotian.(2011).Responses of the growth and biochemical composition of Prorocentrum donghaiense to different nitrogen and phosphorus concentrations.JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY,405(1-2),6-17. |
| MLA | Lai, Junxiang,et al."Responses of the growth and biochemical composition of Prorocentrum donghaiense to different nitrogen and phosphorus concentrations".JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY 405.1-2(2011):6-17. |
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