Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
| Lysozyme as sensitive reporter for fluorometric and PCR based detection of E-coli and S-aureus using magnetic microbeads | |
Zeng, Yan1,2 ; Wan, Yi1; Zhang, Dun1
| |
| 2016-02-01 | |
| 发表期刊 | MICROCHIMICA ACTA
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| 卷号 | 183期号:2页码:741-748 |
| 文章类型 | Article |
| 摘要 | We describe the use of lysozyme as a sensitive reporter for detection of bacteria. The assay comprises the following steps: First, specific primers are used for targeting the rDNA of E. coli and S. aureus, respectively, and the polymerase chain reaction is applied to amplify bacterial DNA. Then, the target DNA binds to the capture probe immobilized on magnetic beads, with lysozyme acting as signal reporter that catalyzes the hydrolysis of an enzyme substrate to form the blue fluorescent product 4-methylumbelliferone. Coupled with multiple lysozyme substrates, this fluorometric detecting method has a detection limit of 50 CFUa <...mL(-1) bacteria. The optical changes may also be detected by colorimetry and monitored with bare eyes. The method was selective over other bacteria and was successfully applied to the determination of E. coli and S. aureus in human serum, which provides a perspective for lysozyme application and species-specific pathogenic bacteria detection. |
| 关键词 | Bacterial Detection Fluorescence Colorimetry Bare Eye Detection Polymerase Chain Reaction Magnetic Beads |
| DOI | 10.1007/s00604-015-1715-1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000370176500028 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/112471 |
| 专题 | 海洋环境腐蚀与生物污损重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Marine Environm Corros & Biofouling, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zeng, Yan,Wan, Yi,Zhang, Dun. Lysozyme as sensitive reporter for fluorometric and PCR based detection of E-coli and S-aureus using magnetic microbeads[J]. MICROCHIMICA ACTA,2016,183(2):741-748. |
| APA | Zeng, Yan,Wan, Yi,&Zhang, Dun.(2016).Lysozyme as sensitive reporter for fluorometric and PCR based detection of E-coli and S-aureus using magnetic microbeads.MICROCHIMICA ACTA,183(2),741-748. |
| MLA | Zeng, Yan,et al."Lysozyme as sensitive reporter for fluorometric and PCR based detection of E-coli and S-aureus using magnetic microbeads".MICROCHIMICA ACTA 183.2(2016):741-748. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Lysozyme as sensitiv（2077KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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