| Functional balance between enzymes in malonyl-CoA pathway for 3-hydroxypropionate biosynthesis | |
| Liu, Changshui1,2; Ding, Yamei3; Zhang, Rubing1; Liu, Huizhou1; Xian, Mo1; Zhao, Guang1 | |
| 2016-03-01 | |
| 发表期刊 | METABOLIC ENGINEERING
 |
| 卷号 | 34期号:2016页码:104-111 |
| 文章类型 | Article |
| 摘要 | 3-Hydroxypropionate (3HP) is an important platform chemical, and four 3HP biosynthetic routes were reported, in which the malonyl-CoA pathway has some expected advantages but presented the lowest 3HP yield. Here, we demonstrated that this low yield was caused by a serious functional imbalance between MCR-C and MCR-N proteins, responsible for the two-step reduction of malonyl-CoA to 3HP. Then we minimized the enzyme activity imbalance by directed evolution of rate-limiting enzyme MCR-C and fine tuning of MCR-N expression level. Combined with culture conditions optimization, our engineering approaches increased the 3HP titer 270-fold, from 0.15 g/L to 40.6 g/L, representing the highest 3HP production via malonyl-CoA pathway so far. This study not only significantly improved the 3HP productivity of recombinant Escherichia coli strain, but also proved the importance of metabolic balance in a multistep biosynthetic pathway, which should be always considered in any metabolic engineering study. (C) 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved. |
| 关键词 | 3-hydroxypropionate Malonyl-coa Pathway Malonyl-coa Reductase Enzymatic Activity Imbalance RE.ombinant E. Coli |
| DOI | 10.1016/j.ymben.2016.01.001 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000369520200010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/112441 |
| 专题 | 海洋环境工程技术研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Changshui,Ding, Yamei,Zhang, Rubing,et al. Functional balance between enzymes in malonyl-CoA pathway for 3-hydroxypropionate biosynthesis[J]. METABOLIC ENGINEERING,2016,34(2016):104-111. |
| APA | Liu, Changshui,Ding, Yamei,Zhang, Rubing,Liu, Huizhou,Xian, Mo,&Zhao, Guang.(2016).Functional balance between enzymes in malonyl-CoA pathway for 3-hydroxypropionate biosynthesis.METABOLIC ENGINEERING,34(2016),104-111. |
| MLA | Liu, Changshui,et al."Functional balance between enzymes in malonyl-CoA pathway for 3-hydroxypropionate biosynthesis".METABOLIC ENGINEERING 34.2016(2016):104-111. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Functional balance b（1232KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论